RNAase protection assay

RNAase protection assay
Sensitive and quantitative alternative to Northern blots for the measurement of gene expression levels. Labelled antisense cRNA is transcribed from a DNA clone in an appropriate vector, hybridized with an mRNA sample, and single stranded RNA digested away with RNAase, then run out on a gel. The amount of labelled RNA surviving is directly proportional to the amount of target mRNA present in the sample.

Dictionary of molecular biology. 2004.

Поможем студентам написать доклад

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”